cy5-labeled ovalbumin in solution  (CompuCyte Corporation)

Journal: PLoS ONE

Article Title: Direct CNS delivery of proteins using thermosensitive liposome-in-gel carrier by heterotopic mucosal engrafting

doi: 10.1371/journal.pone.0208122

Figure Lengend Snippet: (A) Imaging cytometry quantified data from the four selected regions for untreated and Cy5-labeled ovalbumin in saline treated rats (n = 1) using equation total Cy5 intensity = area of Cy5 in selected region in each section * total Cy5 intensity in each selected region in each section. (B) Rat brain cut into 4 equal parts for ELISA with part 3 being the craniotomy site. (C) Ovalbumin detected by ELISA for treatment groups: Untreated (n = 2), ovalbumin saline 48 hours (n = 2), ovalbumin saline 72 hours (n = 2) in the 4 isolated parts of the brain. A 2-way 3 x 4 between and within subjects Anova indicated a significant overall interaction (**** p<0.0001, F (6,9) = 35.75) between different parts (4 brain parts) of the brain and the different treatment groups (untreated, ovalbumin in saline 48 and 72 hours). Post hoc tukey’s tests were further applied to determine differences in ovalbumin uptake in different brain parts. Significant group effects were only found in part 3 where Ovalbumin in saline 48 hours showed significantly greater uptake as compared to untreated rats (** p = 0.0011, DF = 3) and ovalbumin in saline 72 hours(** p = 0.0012, DF = 3). (D) Total ovalbumin (four parts combined) detected by ELISA for treatment groups: Untreated ovalbumin saline 48 hours, ovalbumin saline 72 hours. Ordinary between subjects one way Anova with Tukey’s tests was applied. Ovalbumin in saline (48 hours) showed significantly greater uptake as compared to untreated (*** p = 0.0009, DF = 3) and ovalbumin in saline (72 hours) (** p = 0.0011, DF = 3). A sample size of n = 2 was used for each treatment group.

Article Snippet: The uptake of the Cy5-labeled ovalbumin in solution was quantitatively determined after 72 hours using an ICYTE imaging cytometer (CompuCyte Corp., Cambridge, MA).

Techniques: Imaging, Cytometry, Labeling, Enzyme-linked Immunosorbent Assay, Isolation

Journal: PLoS ONE

Article Title: Direct CNS delivery of proteins using thermosensitive liposome-in-gel carrier by heterotopic mucosal engrafting

doi: 10.1371/journal.pone.0208122

Figure Lengend Snippet: Characterization data (average size, PDI, surface charge and percent encapsulation efficiency) for anionic cy5-labeled ovalbumin liposomes, cationic Cy5-labeled ovalbumin and cationic unlabeled ovalbumin liposomes (n = 3).

Article Snippet: The uptake of the Cy5-labeled ovalbumin in solution was quantitatively determined after 72 hours using an ICYTE imaging cytometer (CompuCyte Corp., Cambridge, MA).

Techniques:

Journal: PLoS ONE

Article Title: Direct CNS delivery of proteins using thermosensitive liposome-in-gel carrier by heterotopic mucosal engrafting

doi: 10.1371/journal.pone.0208122

Figure Lengend Snippet: (A) Anionic Cy5-labeled ovalbumin liposomes (B) cationic Cy5-labeled ovalbumin liposomes (C) and cationic unlabeled ovalbumin liposomes (D) A 30% (w/v) thermo-sensitive Pluronic F-127 solution at 4°C and gel at room temperature.

Article Snippet: The uptake of the Cy5-labeled ovalbumin in solution was quantitatively determined after 72 hours using an ICYTE imaging cytometer (CompuCyte Corp., Cambridge, MA).

Techniques: Labeling

Journal: PLoS ONE

Article Title: Direct CNS delivery of proteins using thermosensitive liposome-in-gel carrier by heterotopic mucosal engrafting

doi: 10.1371/journal.pone.0208122

Figure Lengend Snippet: (A) ICYTE quantified data from the four selected regions for untreated, Cy5- labeled ovalbumin in Pluronic F-127 gel, Cy5-labeled ovalbumin anionic LiG and Cy5-labeled ovalbumin cationic LiG (n = 1) using equation total Cy5 intensity = area of Cy5 in selected region in each section * total Cy5 intensity in each selected region in each section. (B) Ovalbumin detected by ELISA for treatment groups: Untreated (n = 2), ovalbumin LiG 48 hours (n = 2), ovalbumin LiG 72 hours (n = 2) in the 4 isolated parts of the brain. A 3 x 4 between and within subjects 2 way Anova was applied and a significant overall interaction (**** p<0.0001, F (6,9) = 20.15) was found between different parts (4 brain parts) of the brain and the different treatment groups (untreated, ovalbumin in LiG 48 and 72 hours). A post hoc tukey’s test was further applied to determine difference in ovalbumin uptake in different brain parts. Ovalbumin LiG 48 hours shows significant uptake in part 2 as compared to untreated (* p = 0.0154, DF = 3) and ovalbumin LiG 72 hours (* p = 0.0217, DF = 3). Ovalbumin LiG 48 hours also shows significant uptake in part 4 as compared to untreated (** p = 0.0028, DF = 3) and ovalbumin LiG 72 hours (**p = 0.0059, DF = 3). (C) Total Ovalbumin detected (four parts combined) by ELISA for treatment groups: Untreated (n = 2), ovalbumin LiG 48 hours (n = 2), ovalbumin LiG 72 hours (n = 2). Ovalbumin LiG (48 hours) shows significant uptake as compared to untreated (** p = 0.0010, DF = 3) and ovalbumin LiG (72 hours) (** p = 0.0022, DF = 3). (D) Ovalbumin LiG (72 hours) shows significant uptake as compared to untreated (** p = 0.0065, DF = 3) and ovalbumin in saline (72 hours) (* p = 0.0147, DF = 3). A sample size of n = 2 was used for each treatment group.

Article Snippet: The uptake of the Cy5-labeled ovalbumin in solution was quantitatively determined after 72 hours using an ICYTE imaging cytometer (CompuCyte Corp., Cambridge, MA).

Techniques: Labeling, Enzyme-linked Immunosorbent Assay, Isolation

Journal: PLoS ONE

Article Title: Direct CNS delivery of proteins using thermosensitive liposome-in-gel carrier by heterotopic mucosal engrafting

doi: 10.1371/journal.pone.0208122

Figure Lengend Snippet: A 2x6 multifactor repeated measures anova design was applied and a significant overall interaction between the timepoints and vehicles (cy5-labeled ovalbumin in saline and LiG) was obtained (* p<0.05, F (5,5) = 8.349). Post hoc paired T tests were applied to determine the difference between vehicles at each time point. No significant difference was found.A sample size of n = 2 was used for each treatment group.

Article Snippet: The uptake of the Cy5-labeled ovalbumin in solution was quantitatively determined after 72 hours using an ICYTE imaging cytometer (CompuCyte Corp., Cambridge, MA).

Techniques: Labeling